​Mission Statement; My goal is to change the entire Cell Cryopreservation Industry and to help conserve the endangered animals on our planet. 

The sap just works. I have seen what it can do and Mother Nature got it right. 

I  have learned a lot since developing this patent just over 6 years ago. Key information on how to best commercialize the patents, how to use the sap, how to open up new markets, and the logistics of the supply chain are some of the things that I will share with the group that purchases them. 

Mother Nature provides us with at least 22 species of trees that we can utilize. The sap varies in sugar content and it also has other chemicals that show protective properties. These chemicals need to be fully identified. How they protect the plant and animal cells, when frozen, needs to be investigated.  
 
Different species of trees are using chemicals that prevent cell damage in the live layer of the bark surrounding the the entire plant. These same chemicals are now protecting semen cells that are frozen in liquid nitrogen. 

Small Businesses lead the way in developing the science and growth behind their product lines. ​Meet with me online, using Skype. This will allow me to show some incredible videos of semen preserved with the CryoSaps protocol. 
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Funding for Research; Can be obtained through SAM (System for Awards Management in the United States) and Grants.gov. Private enterprise must register with SAM first to be able to apply for the SBIR and STTR grants that are offered through Grants.gov. First obtain a DUNS number and then register with SAM. SBIR grants are the first grants that businesses can apply for. Once proof of concept is demonstrated with the SBIR grants, then applicants are allowed to apply for the larger developmental STTR grants. SBIR grants provide up to $100,000 for research. The STTR grants are larger. The help desks at SAM and Grants.gov are wonderful and well informed about how to do the registrations when you have questions. 
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Advantages of using CryoSaps; 
​The Semen cells are Honest. They live or die based on small changes in the liquid environment that we house them in. These cells will guide the research. 

Freezes in a glass-like state;  Freezing cells in a mixture that freezes in a glass like state is the Holy Grail of Cryopreservation. This will allow you to reduce the toxic cryoprotectants in your mix. 

Improved cell quality on thaw. 

High cell survival rates are being seen using the CryoSaps Protocol. Initially No glycerol or other conventional cryoprotectant was added in. The cell survival rates were impressive. Adding in your IP to the recipe will create a value added product.

Non-animal Origin Cryoprotectant; We provide a non-animal origin cryoprotectant. It does not have the potential to carry pathogens that might come from animal source proteins that are often used to enhance semen cryopreservation. This eliminates the risk of disease spread. 

Does the mix contain antibiotic properties? that may allow producers to refrain from adding antibiotics into their mix? Thus avoiding the ban of semen sales internationally, if the talked about ban of antibiotics in semen straws occurs. 
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Observations;
​I have personally seen avian semen cells survive the freezing in liquid nitrogen using the novel CryoSaps freezing protocol in very high percentages. Avian semen cells are the toughest cells to freeze due to their long thin shape. Their large surface area and small inflexible head size makes these cells very subject to freezing damage. [No glycerol or egg yolk was used].

NO Agglutination observed in Avian semen samples held in the CryoSaps recipe for 72 hours in the fridge at 5 C. There was no head to head, no tail to tail, and no head to tail, agglutination. Similarly, bovine semen cells also housed in this suspension also showed no agglutination. Bovine semen also showed no agglutination when handled the same way.  
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Supplier for dry ingredients;
Morris Livestock Products Company contact information;
​www.morrislivestockproducts.com, P.O. Box 53, Delavan, WI  53115. Phone 262-745-2159 or email at [email protected]. 

​A Liquid Nitrogen Tank is Filled with Liquid Nitrogen. 

Semen is frozen in liquid nitrogen at a​  -198 F. The tank is being filled in this picture from a bulk tank of liquid nitrogen. 

Avian Semen in a Capillary Tube.

Avian semen is collected in a capillary tube prior to freezing. 

Avian Semen under the microscope at 400 X.

Avian semen is the hardest semen to freeze in liquid nitrogen. Its long thin shape makes it very subject to freezing damage. 

A chick hatches. The egg tooth can be seen on the tip of its beak.
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Watch the video link below.

​Our ultimate goal is pictured in the video created by the Poultry CRC and AXS Biomedical Animation Studio Inc. It shows in amazing detail what happens as the embryo develops inside the egg. ​
https://www.youtube.com/watch?v=PedajVADLGw